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Statistical Optimizations of Fermenta...

Statistical Optimizations of Fermentation Factors on Bioethanol Production from Mahua Flower (Madhuca indica) with Saccharomyces cerevisiae by Response Surface Methodology in Batch Bioreactor

Submission Date October,5,2015
Acceptance Date               March,02,2016
Publishing Date March 29,2016
Type of Paper Research Article
Mode of Access Open Access
Author 1. Dr.Dovari.Surndra Nadh Benarji 2. Prof. Chityala Ayyanna
Affiliation 1. Department of Biotechnology,Acharya Nagarjun University,Guntur,522510 2. Director Department of Biotechnology,Al-Ameer College of Engineering and Information Technology Vizag,5310137
Country India 
Contact dsnbenerji@gmail.com

Abstract

The aim of the present investigation is to enhance the bioethanol production from agricultural feed stocks through fermentation process. To bring out facts, experiments were conducted on the biochemical analysis of Mahua flower (Madhuca indica) for its suitability as a raw material and optimizations of fermentation conditions using response surface methodology. The results of fermentation processes on bioethanol productions were compared and tabulated. From the data, it was observed that Saccharomyces cerevisiae-3190 NCIM is high bioethanol tolerant, acid resistant, and is able to produce high yields of bioethanol in high gravity medium. The optimum conditions such as pH 4.9, temperature 31.43 0C, agitation 117.28 RPM, and ammonium sulphate (NH4)2SO4) of 0.629 mg.l-1 were obtained on bioethanol production with statistical optimizations. The yeast strain could produce 150.562g.l-1 and 195.284 g.l-1 with the substrate concentration of 360 g.l-1 and 409 g.l-1 after 48 hours by medium-I and medium-II respectively.

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