|Submission Date||Dec 21,2015|
|Publishing Date||May 10,2016|
|Type of Paper||Research Article|
|Mode of Access||Open Access|
|Author||Dasarathi Das*, Prakasini Satapathy, Biswanath Murmu|
|Affiliation||Tuberculosis Division, Regional Medical Research Centre*, South Eastern Railways Complex, Bhubaneswar-751023, India|
Sputum decontamination by modified Petroff’s method is routinely used by many laboratories for isolation of Mycobacteria in solid culture. With the introduction of line probe assay (LPA) for MDR TB diagnosis, our laboratory adopted the recommended NALC–NaOH decontamination procedure for sputum processing. Out of 284 and 1039 specimens processed by modified Petroff’s and NALC-NaOH, significantly high percentage (P=0.004) of contamination was observed by the latter method. In the initial period, the contamination reached 19.5% with the NALC-NaOH method and reduced with time. Laboratories adopting new decontamination methods may be careful during the initial months for avoiding the loss of specimen due to contamination while adopting LPA.