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Shifting from modified Petroff’s to N...

Shifting from modified Petroff’s to NALC-NaOH method for processing of sputum specimens for solid culture

Submission Date Dec 21,2015
Acceptance Date Apr,16,2016
Publishing Date May 10,2016
Type of Paper Research Article
Mode of Access Open Access
Author Dasarathi Das*, Prakasini Satapathy, Biswanath Murmu
Affiliation Tuberculosis Division, Regional Medical Research Centre*, South Eastern Railways Complex, Bhubaneswar-751023, India
Country India 
Contact drdas60@rediffmail.com

Abstract

Sputum decontamination by modified Petroff’s method is routinely used by many laboratories for isolation of Mycobacteria in solid culture. With the introduction of line probe assay (LPA) for MDR TB diagnosis, our laboratory adopted the recommended NALC–NaOH decontamination procedure for sputum processing. Out of 284 and 1039 specimens processed by modified Petroff’s and NALC-NaOH, significantly high percentage (P=0.004) of contamination was observed by the latter method. In the initial period, the contamination reached 19.5% with the NALC-NaOH method and reduced with time. Laboratories adopting new decontamination methods may be careful during the initial months for avoiding the loss of specimen due to contamination while adopting LPA.

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