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Serological and virological profile o...

Serological and virological profile of dengue fever in a tertiary care hospital, southern part of Hyderabad, during 2011-12

Submission Date : Sat, Apr 18, 2015
Acceptance Date : Thu, May 07, 2015
Publishing Date : Mon,18,2015
Type of Paper : Research Article
Mode of Access : Open Access
Author : * Swathi Akula 1, Nagamani Kammili 3
Affiliation :1 .Assistant professor, Department of Microbiology, KAMS&RC, LB Nagar, Hyderabad, Telangana 500068, India.2 India3. Department of Microbiology, Gandhi Medical College, Secunderabad 500034, India
Country :India
Contact :swathi.sho@gmail.com

Abstract

Dengue fever is endemic in India. Andhra Pradesh is one of the hotspots for dengue but an understanding of the incidence of the disease as well the prevailing serotypes of dengue virus is lacking.The study was conducted to determine the prevalence of dengue syndrome among patients admitted with clinical suspicion of dengue, the clinical, demographic profile, serological profile of dengue syndrome, and to identify the dengue virus serotypes prevalent in the year 2011 and 2012. Samples (n = 237) with clinical suspicion of dengue fever were tested for the presence of dengue NS1 antigen (NS1Ag) as well as DENV-specific IgM and IgG antibodies by ELISA. Serum samples were subjected to cell culture followed by RT-PCR using group-specific and type-specific primers. The seroprevalance of dengue syndrome was 35% among the study samples. IgM ELISA and NS1Ag ELISA detected 68.6% and 43.3% of the cases, respectively. NS1Ag could detect 10% of the cases that were negative by IgM ELISA in the early pyrexic stage. Primary and secondary dengue fever was more frequent in children (0-10 yr) and young adults (21-30 yr), with a frequency of 59% and 45.9%, respectively. Five samples from 2011 contained DENV1 serotype, and ten samples from 2012 indicated the co-circulation of DENV1 and DENV3 serotypes. Hemorrhagic manifestation was more common in patients having co-infections.

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